FLOWJO SOFTWARE HOW TO

The workflows also contain steps for dimensionality reduction using tSNE and UMAP, including visualizations of the results.Įach contains an additional heatmap, created using MEM Score values, that allows the evaluation of the characteristics of the flowSOM clusters. They contain steps to perform a standard clustering analysis using self-organizing maps (flowSOM) and have visualizations of the clusters in Heatmap and Minimum Spanning Tree formats. Note: Due to updates the names of available templates may be slightly different to the ones shown in the images here. Removing abnormal data points, debris, doublets and dead cells in advance will speed up the uploading process and help you get a more accurate analysis.Īfter performing your preferred cleaning and gating strategy in FlowJo select a population of your interest by clicking on it with your mouse.

We recommend you QC and clean your data with FlowJo tools before uploading to Tercen.

FLOWJO SOFTWARE HOW TO

This guide does not attempt to show you how to use FlowJo software and we recommend you consult the FlowJo website for user manuals and tutorials. Upload from FlowJo to Tercen Step 1: Select a population. The Tercen plugin will now be listed in the Plugins drop-down menu. Quit and restart FlowJo to activate the plugin. Note: Delete any older versions of the Tercen plugin that you may have previously installed. Refer to the FlowJo Plugins Documentation to set up or locate your plugins folder. The location of the plugin folder differs depending on your operation system. Tercen_v1.1.1.jar) to the FlowJo plugins folder. To install the Tercen plugin, move or copy the Tercen plugin file (e.g. Step 2: Add the Tercen plugin file to your FlowJo plugin folder This PDF contains installation instructions. Your Tercen plugin file may have different version numbers). Open the downloaded folder and extract the files. Step 1: Download the Tercen plugin folder and extract its contents.

Flowjo software